Determination of Volatile Fatty Acids (VFA ) in Silage – Gas Chromatography

1. Principle A water extract from silage is acidified and centrifuged. The alcohols and volatile fatty acids (VFA) are separated in the chromatography column depending on their molecular weight and then detected, identified, amplified, and areas integrated. 2. Scope The procedure for determination of alcohols and volatile fatty acids is only applicable to silages. 3….

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Urea – Spectrophotometric Method

1. Principle The sample (feed or feed ingredient) is suspended in distilled water with a clarifying agent. The urea content is determined using a spectrophotometer after addition of 4-dimethylaminobenzaldehyde (4-DMAB). 2. Scope The method is suitable for feeds and forages. Some feeds have added urea as a supplemental source of nitrogen. This method describes determination…

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Nutrition for body maintenance; growth, production and reproduction in ruminants

Nutrition plays a critical role in the health, productivity, and overall performance of ruminants. Ruminant animals, such as cattle, sheep, goats, and buffalo, have a unique digestive system that allows them to efficiently process fibrous plant material. Their nutritional needs vary depending on the stage of life, production status, and environmental conditions. Proper nutrition is…

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Effect of over and underfeeding in livestock and poultry and its economic importance

Overfeeding and underfeeding livestock and poultry can have significant impacts on their health, productivity, and overall profitability. The effects on animal performance and the economic importance of these issues are crucial for farmers and producers to understand in order to make informed decisions about nutrition, feeding practices, and resource management. Effect of Overfeeding in Livestock…

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Use of spectrophotometer for analysis of minerals elements

Spectrophotometry is a powerful analytical technique used to measure the concentration of mineral elements in biological samples, such as animal feed, tissues, blood, urine, and other fluids. This technique involves the measurement of light absorption at specific wavelengths, which is directly related to the concentration of mineral elements in the sample. Here’s an advanced procedure…

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Use of Thin Layer Chromatography (TLC)TLC Technique in Animal Nutrition

Thin Layer Chromatography (TLC) is a widely used analytical technique in animal nutrition for separating and identifying compounds present in biological samples, such as feed, food, or animal tissues. The application of TLC in animal nutrition includes: 1. Identification of Nutrients: TLC can be used to separate and identify various nutrients in animal feeds, such…

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Vitamins: Role, clinical Sign and requirement

Vitamins Vitamin A Role of Vitamin A • Synthesis of glycoprotein to maintain integrity of epithelial cells. • In bone formation synthesis of mucopolysaccharides. • Synthesis of the visual pigment Rhodopsin. • Retinol and retinoic acid (RA) are essential for embryonic development during fetal development. Clinical signs • Inadequate retinol available to the retina results…

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