Animal / Poultry Nutrition
Determination of Ether Extract/Fat
The dried feed sample is dissolved with petrol ether and then the ether is evaporated in the Soxhlet apparatus. The residues are crude fat. In the Animal Nutrition Laboratory at ICARDA a Soxtherm apparatus from Gerhardt GmbH is used. The
Determination of Crude Fiber – Filtration Method
1. Principle The sample after defatting is sequentially treated with boiling dilute sulphuric acid, and with boiling potassium hydroxide solution. The loss in mass resulting from incineration corresponds to the mass of crude fiber. 2. Scope The method described is
Determination of Nitrogen and Calculation of Crude Protein – Kjeldahl
1. Principle For determination of nitrogen the sample is digested using sulphuric acid in the presence Of a catalyst to convert sample nitrogen to ammonium sulphate. The acid solution is made Alkaline with sodium hydroxide solution. The ammonia is distilled
Determination of Crude Ash
1. Principle Ash is determined gravimetrically as the residue after incineration at 550 °C. 2. Scope This procedure is applicable for determination of ash in feed ingredients and feeds. This method cannot be used for mineral mixtures. 3. Responsibilities Laboratory
Laboratory Safety
Laboratory Neatness Clean and neat work areas avoid risk of damage to clothing and books and injury from spilled chemicals. Neatness also reduces fire hazard. Laboratory Conduct Fooling around in the laboratory can be hazardous. Keep the lab in its