Animal / Poultry Nutrition
Determination of Acid Detergent Fiber (ADF)
Principle Acid detergent fiber is determined as the residue remaining after adding an acidified solution. It is the NDF without the hemicelluloses. Cell soluble, hemicelluloses and soluble minerals dissolve. Cetyl trimethyl-ammonium bromide (CTAB) separates proteins from the remaining cellulose and
Metabolic antagonistics and incompatibilities in mixed feed of poultry
Metabolic antagonisms and incompatibilities can occur in mixed feed for poultry when certain nutrients or feed ingredients interact negatively, impairing nutrient absorption, utilization, or metabolic processes. Here are some examples of metabolic antagonisms and incompatibilities in mixed feed for poultry:
Determination of Neutral Detergent Fiber (NDF)
Principle Insoluble fiber in feed is determined as neutral detergent fiber (NDF). Neutral detergent solution recovers its main components cellulose, hemicelluloses and lignin. Procedure • Weigh oven-dry glass crucible (Wt) • Add 0.5–1 g sample (Ws) in 600 ml Berzelius
Determination of Ether Extract/Fat
The dried feed sample is dissolved with petrol ether and then the ether is evaporated in the Soxhlet apparatus. The residues are crude fat. In the Animal Nutrition Laboratory at ICARDA a Soxtherm apparatus from Gerhardt GmbH is used. The
Determination of Crude Fiber – Filtration Method
1. Principle The sample after defatting is sequentially treated with boiling dilute sulphuric acid, and with boiling potassium hydroxide solution. The loss in mass resulting from incineration corresponds to the mass of crude fiber. 2. Scope The method described is
Determination of Nitrogen and Calculation of Crude Protein – Kjeldahl
1. Principle For determination of nitrogen the sample is digested using sulphuric acid in the presence Of a catalyst to convert sample nitrogen to ammonium sulphate. The acid solution is made Alkaline with sodium hydroxide solution. The ammonia is distilled