Animal/Poltry Nutrition

Animal / Poultry Nutrition

Determination of Acid Detergent Fiber (ADF)

Principle Acid detergent fiber is determined as the residue remaining after adding an acidified solution. It is the NDF without the hemicelluloses. Cell soluble, hemicelluloses and soluble minerals dissolve. Cetyl trimethyl-ammonium bromide (CTAB) separates proteins from the remaining cellulose and

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Determination of Neutral Detergent Fiber (NDF)

Principle Insoluble fiber in feed is determined as neutral detergent fiber (NDF). Neutral detergent solution recovers its main components cellulose, hemicelluloses and lignin. Procedure • Weigh oven-dry glass crucible (Wt) • Add 0.5–1 g sample (Ws) in 600 ml Berzelius

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Determination of Ether Extract/Fat

The dried feed sample is dissolved with petrol ether and then the ether is evaporated in the Soxhlet apparatus. The residues are crude fat. In the Animal Nutrition Laboratory at ICARDA a Soxtherm apparatus from Gerhardt GmbH is used. The

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Determination of Crude Fiber – Filtration Method

1. Principle The sample after defatting is sequentially treated with boiling dilute sulphuric acid, and with boiling potassium hydroxide solution. The loss in mass resulting from incineration corresponds to the mass of crude fiber. 2. Scope The method described is

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