Animal/Poltry Nutrition

Animal / Poultry Nutrition

Determination of Gross Energy

1. Principle Gross energy is measured by combustion of a sample in an excess of oxygen in a bomb calorimeter under standardized conditions. The gross calorific value is calculated from the temperature rise of the water in the calorimeter vessel

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Thin Layer Chromatography Procedure

1) Apparatus:- Ultraviolet light (UV), glass plates, 4×8, spreader , oven ,micro pipette, beaker ,shaker ,electric balance, water bath, china dish 2) Reagents:- Silica gel (powder form) 254 Gr 60 number, double dish H2O, chloroform, standard solution, 3) Procedure:- Sample

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Urea – Spectrophotometric Method

1. Principle The sample (feed or feed ingredient) is suspended in distilled water with a clarifying agent. The urea content is determined using a spectrophotometer after addition of 4-dimethylaminobenzaldehyde (4-DMAB). 2. Scope The method is suitable for feeds and forages.

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