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Branched chain amino acids

Isoleucine, leucine and valine are the three branched chain amino acids. Their metabolism is unique, sharing the same catabolism pathway and therefore interfere with each other. Leucine is a strong regulator of the branched chain amino acid catabolism. As a consequence, minimum supply of valine and isoleucine must be ensured in the diet and an…

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Protein quality

Protein is not a nutrient. Animals require amino acids and not protein per se. The quality of protein supply is determined by its potential to cover the physiological requirements in terms of amino acids for maintenance and performance (growth, reproduction, production of milk and eggs). The quality of protein required is different depending on the…

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Amino acids Chemical structure

Amino acids are characterised by the two characteristic functional groups in the molecule, as indicated by their nomenclature, by the amino group NH2 and the carboxyl group COOH. Amino acids occur exclusively as structural protein units in which the amino group is bound to the α-position of the carboxylic acid group (carboxyl group). Chemically amino…

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Amino Acids

The need to feed a growing population leads to a trend for highly specialised units where animals of high genetic merit are raised in optimum conditions. Advances in husbandry techniques are also made in response to the needs for rearing high genetic merit livestock. Improvements in feed efficiency which have been gained over the last…

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Splitting up into different evolutionary lines

The diversification of insects into different evolutionary lines and their success in occupying diverse environments can be attributed to several key factors. These factors include their evolutionary adaptations, unique biological features, and environmental influences that have allowed them to thrive in nearly every ecosystem on Earth. 1. Splitting into Different Evolutionary Lines: The diversification of…

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Determination of Gross Energy

1. Principle Gross energy is measured by combustion of a sample in an excess of oxygen in a bomb calorimeter under standardized conditions. The gross calorific value is calculated from the temperature rise of the water in the calorimeter vessel and the mean effective heat capacity of the calorimeter. 2. Scope This method is applicable…

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Determination of Volatile Fatty Acids (VFA ) in Silage – Gas Chromatography

1. Principle A water extract from silage is acidified and centrifuged. The alcohols and volatile fatty acids (VFA) are separated in the chromatography column depending on their molecular weight and then detected, identified, amplified, and areas integrated. 2. Scope The procedure for determination of alcohols and volatile fatty acids is only applicable to silages. 3….

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Thin Layer Chromatography Procedure

1) Apparatus:- Ultraviolet light (UV), glass plates, 4×8, spreader , oven ,micro pipette, beaker ,shaker ,electric balance, water bath, china dish 2) Reagents:- Silica gel (powder form) 254 Gr 60 number, double dish H2O, chloroform, standard solution, 3) Procedure:- Sample preparation:- a) Take 25 gm sample add 20 ml distilled water. b) Mix it and…

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Urea – Spectrophotometric Method

1. Principle The sample (feed or feed ingredient) is suspended in distilled water with a clarifying agent. The urea content is determined using a spectrophotometer after addition of 4-dimethylaminobenzaldehyde (4-DMAB). 2. Scope The method is suitable for feeds and forages. Some feeds have added urea as a supplemental source of nitrogen. This method describes determination…

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Nutrition for body maintenance; growth, production and reproduction in ruminants

Nutrition plays a critical role in the health, productivity, and overall performance of ruminants. Ruminant animals, such as cattle, sheep, goats, and buffalo, have a unique digestive system that allows them to efficiently process fibrous plant material. Their nutritional needs vary depending on the stage of life, production status, and environmental conditions. Proper nutrition is…

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