Demonstration for the proper use of microscope

Microscope

A microscope is a laboratory instrument use to examine objects that are too small to be seen with naked eye.

Types of microscope

Stereoscope (or stereo microscope)

A stereo microscope on the other hand is generally used to inspect larger objects such as small mechanical pieces, minerals, insects and more.

Compound microscope

A compound microscope is generally used to view very small specimens or objects that you wouldn’t normally see with the naked eye.

Using a microscope correctly is essential for obtaining clear and accurate observations of specimens. Whether you are using a light microscope for biological samples or an electron microscope for more detailed imaging, the basic principles of proper microscope use remain consistent. Here are general guidelines for the proper use of a light microscope:

1. Setting Up the Microscope:

Clean the Lenses: Ensure that the lenses (objective and eyepiece) are clean using lens paper or a gentle lens-cleaning solution.

Turn on the Illumination: If using a compound microscope, turn on the light source.

2. Adjusting the Illumination:

Adjust the Light Intensity: Use the light intensity control to achieve proper illumination without overexposing the specimen.

Köhler Illumination (if applicable): Align the microscope for Köhler illumination to ensure even lighting across the field of view.

3. Setting the Stage:

Place the Specimen: Put the specimen on the microscope stage and secure it with stage clips or a mechanical stage, if available.

Choose the Objective: Begin with the lowest magnification objective for initial focusing.

4. Focusing:

Coarse Adjustment: Use the coarse adjustment knob to bring the specimen into view. Move the objective away from the specimen before using the coarse adjustment to avoid contact.

Fine Adjustment: Refine the focus using the fine adjustment knob for sharp detail.

5. Changing Objectives:

Rotate the Turret: If using a compound microscope with multiple objectives, rotate the turret to switch objectives.

Re-focus: After changing objectives, use the fine adjustment knob to bring the new objective into focus.

6. Eyepiece Adjustment:

Adjust the Diopter: If the microscope has a diopter adjustment on one of the eyepieces, adjust it to compensate for any differences in vision between your eyes.

7. Using the Condenser:

Adjust the Condenser Height: Raise or lower the condenser to control the light entering the specimen. For higher magnifications, the condenser is usually raised.

8. Observing and Recording:

Look Through the Eyepiece: Observe the specimen through the eyepiece and make any necessary adjustments for clarity.

Record Observations: If needed, use a camera attachment or smartphone adapter to record images.

9. Cleaning and Shutdown:

Turn Off the Light Source: For compound microscopes, turn off the light source before removing the specimen.

Remove the Specimen: Carefully remove the specimen from the stage.

Clean the Lenses: Clean the lenses with lens paper or a gentle lens-cleaning solution.

Cover or Store: If available, cover the microscope or store it in a designated area.

10. Maintenance:

Regular Maintenance: Follow the manufacturer’s guidelines for regular maintenance, including lubricating moving parts and checking for any issues.

Always refer to the specific instructions provided by the manufacturer for your microscope model, as different microscopes may have specific features and requirements. Additionally, adhere to laboratory safety guidelines and practices when using microscopes, especially if working with biological or chemical specimens.